卵巢上皮性癌中干扰素介导的跨膜蛋白1的表达意义

目的:探讨干扰素介导的跨膜蛋白1(Interferon-induced transmembrane protein 1,IFITM1)基因在卵巢上皮性癌中表达的相关性及其意义。方法:应用Western blotting检测正常卵巢、卵巢良性肿瘤、卵巢交界性肿瘤和卵巢上皮性癌组织中IFITM1蛋白表达。免疫组织化学检测12例正常卵巢、21例卵巢良性肿瘤、18例卵巢交界性肿瘤和85例卵巢上皮性癌组织中IFITM1的蛋白表达,同时分析IFITM1表达状况与临床病理因素之间的相关性。结果:Western blotting显示卵巢上皮性癌和卵巢交界性肿瘤中IFITM1表达水平明显高于正常卵巢组织和卵巢良性肿瘤。免疫组化显示在正常卵巢组织中IFITM1阳性表达率为41.7%(5/12),在卵巢良性肿瘤组织中71.4%(15/21),在卵巢交界性肿瘤组织中为72.2%(13/18),在卵巢上皮性癌中为77.6%(66/85),IFITM1蛋白表达强度在正常卵巢、良性卵巢肿瘤、交界性卵巢肿瘤、上皮性卵巢癌间的比较有统计学意义(P0.05)。IFITM1蛋白表达与病理类型、肿瘤分化程度、肿瘤FIGO分期有关(P0.05),与淋巴结转移、腹水无明显相关性。化疗敏感组和耐药组的IFITM1表达强度间差异有统计学意义(P0.05)。结论:IFITM1在正常卵巢、卵巢良性肿瘤、卵巢交界性肿瘤和卵巢上皮性癌组织中的表达依次升高,并与卵巢癌以铂类为基础的化疗耐药性产生有相关性,为进一步研究IFITM1在卵巢癌诊治及化疗中的应用前景提供依据。

Expression and Significance of IFITM1 in Epithelial Ovarian Carcinoma

Objective:To investigate the role of Interferon-induced transmembrane protein 1 (IFITM1 ) in the carcinogenesis and evaluate its clinical significance in epithelial ovarian carcinoma.Methods:The expression level of IFITM1 was detected by the Western blot analysis in different ovaries, and the immunohistochemical method (SP) was used to determine the expression levels of IFITM1 among 1 2 normal ovarian tissue cases, 21 benign ovarian tumor cases, 1 8 borderline ovarian tumor cases and 85 ovarian carcinoma cases. Then the relationship between the expression level of IFITM1 in ovarian carcinoma and the clinicopathological features was analyzed by statistics.Results:Western blot analysis showed that the expression levels of IFITM1 differed in different ovarian tissues. The immunohistochemical technique showed that the positive rate of IFITM1 expression was 77.6% (66/85) in ovarian carcinoma, higher than that of ovarian (41 .7 %, 5/12), benign ovarian tumor (71.4 %, 15/21) and borderline ovarian tumor (72.2 %, 13/1 8) with a dramatical statistic significance (P<0.05). The expression level ofIFITM1 was correlated with pathology type, tumor grad and FIGO stage in ovrian carcinoma (P<0.05). The expression intensity of IFITM1 protein in ovarian carcinoma was significantly related to its chemotherapy sensitivity.Conclusion:The expression levels ofIFITM1 gradually increased in normal ovarian tissue, benign ovarian tumor, borderline ovarian tumor and ovarian carcinoma. Positive expression level of IFITM1 played a vital role in carcinogenesis and progression of ovarian carcinoma including the drug-resistance to platinum-based chemotherapy. Our research can provide better evidence to the further study of IFITM1 in diagnosis and targeted treatment of ovarian carcinoma.