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Genetic analysis of cysteine-poor prolamin polypeptides reduced in the endosperm of the rice esp1 mutant |
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| Authors: | Tomokazu Ushijima Hiroaki Matsusaka Hiroyuki Jikuya Masahiro OgawaHikaru Satoh Toshihiro Kumamaru |
| Institution: | a Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Fukuoka 812-8581, Japan b Bio-Architecture Center, Kyushu University, Hakozaki 6-10-1, Fukuoka 812-8581, Japan c Department of Life Science, Yamaguchi Prefectural University, Sakurabatake, Yamaguchi 753-8502, Japan |
| Abstract: | The esp1 mutant CM21 specifically exhibits reduced levels of cysteine-poor (CysP) prolamin bands with pIs of 6.65, 6.95, 7.10, and 7.35 in rice seed. Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis demonstrated that the bands with pIs 6.65, 6.95, and 7.35 are encoded by different structural genes. These results suggest that the Esp1 locus encodes a regulatory factor involved in the synthesis and/or accumulation of CysP prolamin molecules. Isoelectric focusing (IEF) analysis of CysP prolamins in chromosome substitution lines showed that structural genes for bands with pI values of 6.95, 7.10, and 7.35, which are reduced in esp1 mutant lines, are located as a gene cluster in the 44.2 cM region on chromosome 5. |
| Keywords: | MALDI-TOF MS Mutant Prolamin Rice Starchy endosperm Storage protein |
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