Metabolic changes of iron uptake in N2-fixing common bean nodules during iron deficiency |
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Authors: | Tarek Slatni,Gianpiero ViganiImen Ben Salah,Saber KouasMarta Dell&rsquo Orto,Houda GouiaGraziano Zocchi,Chedly Abdelly |
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Affiliation: | a Laboratoire des Plantes Extrêmophiles, Centre de Biotechnologie, Technopark de Borj Cedria, BP 901, Hammam Lif 2050, Tunisia b Unité de Nutrition et Métabolisme Azotés et Protéines de Stress, Département de Biologie, Faculté des Sciences de Tunis, Campus Universitaire, 1060 Tunis, Tunisia c Università Degli Studi Di Milano, Dipartimento di Produzione Vegetale, Sezione di Fisiologia delle Piante Coltivate e Chimica Agraria, Via Celoria 2 - 20133 Milano, Italy |
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Abstract: | Iron is an important nutrient in N2-fixing legume nodules. The demand for this micronutrient increases during the symbiosis establishment, where the metal is utilized for the synthesis of various iron-containing proteins in both the plant and the bacteroid. Unfortunately, in spite of its importance, iron is poorly available to plant uptake since its solubility is very low when in its oxidized form Fe(III). In the present study, the effect of iron deficiency on the activity of some proteins involved in Strategy I response, such as Fe-chelate reductase (FC-R), H+-ATPase, and phosphoenolpyruvate carboxylase (PEPC) and the protein level of iron regulated transporter (IRT1) and H+-ATPase proteins has been investigated in both roots and nodules of a tolerant (Flamingo) and a susceptible (Coco blanc) cultivar of common bean plants. The main results of this study show that the symbiotic tolerance of Flamingo can be ascribed to a greater increase in the FC-R and H+-ATPase activities in both roots and nodules, leading to a more efficient Fe supply to nodulating tissues. The strong increase in PEPC activity and organic acid content, in the Flamingo root nodules, suggests that under iron deficiency nodules can modify their metabolism in order to sustain those activities necessary to acquire Fe directly from the soil solution. |
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Keywords: | Ferric chelate reductase H+-ATPase Iron deficiency Nodules Phosphoenolpyruvate carboxylase |
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