Use of an interspecific hybrid in identifying a new allelic specificity generated at the self-incompatibility locus after inbreeding in Lycopersicon peruvianum |
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Authors: | G Maheswaran T Perryman E G Williams |
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Institution: | (1) Plant Cell Biology Research Centre, School of Botany, University of Melbourne, 3052 Parkville, Victoria, Australia |
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Abstract: | Summary An interspecific hybrid between Lycopersicon esculentum () and L. peruvianum has been raised by embryo rescue in vitro and used to confirm the presence of a new S-allelic specificity in its inbred L. peruvianum parent, a plant derived by enforced bud self-pollination of a self-incompatible clone with the genotype S
1
S
2. The inbred plant showed breeding behavior characteristic of both S
2 and a second specificity which was not S
1, S
2, S
3 or S
f. Two-dimensional gel electrophoresis of stylar proteins, however, showed only a single typical S-associated component with the Mr and pI characteristic of S2. The alteration in specificity, therefore, was not associated with a detectable change in an S-associated protein. The F1 interspecific hybrid showed intermediacy of vegetative and reproductive characters, relatively high fertility and full self-incompatibility. Backcrossing to L. esculentum produced only abortive seeds requiring embryo culture. Backcrosses to L. peruvianum produced a very low proportion of filled germinable seeds. Pollen of the hybrid showed superior viability and tube growth rate compared with pollen of the two parent plants. |
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Keywords: | Self-incompatibility Hybridization Inbreeding Embryo culture Tomato Heterosis Lycopersicon |
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