Valoración de niveles de testosterona mediante diferentes métodos analíticos en varones sanos |
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| Authors: | Concepción Sanabria Pérez José Angel Díaz Pérez |
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| Institution: | 1. Profesor titular de Otorrinolaringología.Facultad de Medicina.La Laguna;2. Servicio de Otorrinolaringología. Departamento de Cirugía. Hospital Universitario de Canarias;3. Servicio de Otorrinolaringología. Departamento de Cirugía. Hospital Universitario Nuestra Señora de Candelaria;4. Servicio de Farmacología Clínica. Departamento de Medicina Física y Farmacología. Hospital Universitario de Canarias;1. Institute of Chemistry and Biochemistry (ICBMS – UMR CNRS 5246), Université de Lyon, Université Lyon 1, CNRS, 43 Bd du 11 novembre 1918, 69622 Lyon, France;2. CERMEP-In vivo imaging, Groupement Hospitalier Est, 59 Bd Pinel, 69003 Lyon, France;3. Lyon Neuroscience Research Center (UMR CNRS 5292, UMR INSERM 1028), Groupement Hospitalier Est, 59 Bd Pinel, 69003 Lyon, France;1. Department of Internal Medicine, ZGT Hospital, Almelo, the Netherlands;2. Department of Internal Medicine, Erasmus Medical Center, Rotterdam, the Netherlands;3. Division of Nephrology, University Hospital Zurich, Zürich, Switzerland;4. Institute of Physiology and Zürich Center for Human Intergrative Physiology, University of Zürich, Zürich, Switzerland;1. Programa de Pós-Graduação em Química, Instituto de Química, Universidade Federal Fluminense, Outeiro de São João Batista s/n°, 24020-141 Niterói, RJ, Brazil;2. Faculdade de Farmácia, Universidade Federal Fluminense, Rua Mário Viana 523, 24241-000 Niterói, RJ, Brazil;3. Laboratório de Oncologia Experimental, Universidade Federal do Ceará, Rua Coronel Nunes de Melo 1127, 60430-270 Fortaleza, CE, Brazil;4. Laboratório de Citogenética Humana, Instituto de Ciências Biológicas, Universidade Federal do Pará, Rua Augusto Corrêa 01, 66075-110 Belém, PA, Brazil;5. Departamento de Química, Instituto de Ciências Exatas, Universidade Federal Fluminense, Rua Des. Ellis Hermydio Figueira 783, 27213-415, Volta Redonda, RJ, Brazil |
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| Abstract: | Plasma testosterone concentrations are essential for the diagnosis of several causes of hypogonadism, including late-onset hypogonadism. Defining the normal range for testosterone concentrations poses certain difficulties due to the changes that occur with age and the variability of the different analytical methods used.ObjectivesTo study normal ranges of testosterone in healthy young men and to compare the results of distinct analytical methods.Material and methodsWe recruited 20 healthy men with a mean age of 24.5 years (standard deviation (SD): 5.04) and a mean body mass index (BMI) of 23.8% (SD: 3.3). Total testosterone (TT) was measured by immunochemiluminescence (ICLA) and free testosterone (FT) by radioimmunoassay (RIA). Calculated free testosterone (FTc) and bioavailable testosterone (BT) were calculated using Vermeulen's formula. Serum lutropin (LH), follitropin (FSH) and sex hormone binding globulin (SHBG) were measured by immunoradiometric assays (IRMA).ResultsThe mean concentrations were 20 nmol/l (SD: 4.96) for TT, 0.054 nmol/L (SD: 0.01) for FT, 0.3834 nmol/L (SD: 0.09) for FTc and 9.9 nmol/L (SD: 2.8) for BT. There was no correlation between testosterone measured by different methods other than an association between FT and FTc (r=0.662, p<0.003) and between FTc and BT (r=0.979, p<0.0001). An inverse correlation was found between BMI and TT concentrations (r: ?0.52, p<0.017).ConclusionsThe normal range for testosterone in healthy young men should be established in each laboratory based on the analytical method used. |
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