Generation of a set of conditional analog-sensitive alleles of essential protein kinases in the fission yeast Schizosaccharomyces pombe |
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| Authors: | Lubos Cipak Chao Zhang Ines Kovacikova Cornelia Rumpf Eva Miadokova Kevan M Shokat Juraj Gregan |
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| Institution: | 1.Max F. Perutz Laboratories; University of Vienna; Vienna, Austria;2.Laboratory of Cancer Genetics; Cancer Research Institute; Slovak Academy of Sciences; Bratislava, Slovak Republic;3.Department of Cellular and Molecular Pharmacology; Howard Hughes Medical Institute; University of California; San Francisco, CA USA;4.Department of Chemistry; University of Southern California, Los Angeles, CA USA;5.Department of Genetics; Comenius University; Bratislava, Slovak Republic |
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| Abstract: | The genome of the fission yeast Schizosaccharomyces pombe encodes for 17 protein kinases that are essential for viability. Studies of the essential kinases often require the use of mutant strains carrying conditional alleles. To inactivate these kinases conditionally, we applied a recently developed chemical genetic strategy. The mutation of a single residue in the ATP-binding pocket confers sensitivity to small-molecule inhibitors, allowing for specific inactivation of the modified kinase. Using this approach, we constructed conditional analog-sensitive alleles of 13 essential protein kinases in the fission yeast S. pombe.Key words: kinase, analog-sensitive, conditional allele, fission yeast, phosphorylation |
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