Interplay of DNA repair,homologous recombination,and DNA polymerases in resistance to the DNA damaging agent 4-nitroquinoline-1-oxide in Escherichia coli |
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| Authors: | Ashley B Williams Kyle M Hetrick Patricia L Foster |
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| Institution: | 1. Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089, United States;2. Department of Biology, Indiana University, 1001 East Third Street, Bloomington, IN 47401, United States;1. Department of Physics and Biophysics, Wroclaw University of Environmental and Life Sciences, ul. Norwida 25, 50-375 Wroc?aw, Poland;2. Faculty of Chemistry, University of Wroc?aw, ul. Joliot-Curie 14, 50-383 Wroc?aw, Poland;1. Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA;2. The Ohio State Biophysics Program, The Ohio State University, Columbus, OH 43210, USA;3. The Ohio State Biochemistry Program, The Ohio State University, Columbus, OH 43210, USA;4. Department of Chemistry, University of Connecticut, Storrs, CT 06269, USA;1. Institute of Systems Biomedicine, Department of Radiation Medicine, School of Basic Medical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, P.R. China;2. Department of Experimental Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA;1. Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Brighton BN1 9RQ, UK;2. Lancaster Medical School, Faculty of Health and Medicine, Lancaster University, Lancaster LA1 4YQ, UK;3. Institute for Research in Biomedicine (IRB Barcelona), Barcelona 08028, Spain |
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| Abstract: | Escherichia coli has three DNA damage-inducible DNA polymerases: DNA polymerase II (Pol II), DNA polymerase IV (Pol IV), and DNA polymerase V (Pol V). While the in vivo function of Pol V is well understood, the precise roles of Pol IV and Pol II in DNA replication and repair are not as clear. Study of these polymerases has largely focused on their participation in the recovery of failed replication forks, translesion DNA synthesis, and origin-independent DNA replication. However, their roles in other repair and recombination pathways in E. coli have not been extensively examined. This study investigated how E. coli's inducible DNA polymerases and various DNA repair and recombination pathways function together to convey resistance to 4-nitroquinoline-1-oxide (NQO), a DNA damaging agent that produces replication blocking DNA base adducts. The data suggest that full resistance to this compound depends upon an intricate interplay among the activities of the inducible DNA polymerases and recombination. The data also suggest new relationships between the different pathways that process recombination intermediates. |
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