不同性质甲状腺结节与微血管密度关系的研究

目的:探讨不同性质甲状腺结节与微血管密度关系,提高认识.方法:分别选取甲状腺乳头状癌、甲状腺腺瘤、结节性甲状腺肿及正常甲状腺组织,病理切片行常规HE染色及免疫组化SP染色.在高倍视野(×400)下选取5个血管着色密集区进行计数并取其平均数,MVD值=(n1 +n2+n3+n4+n5)/5.结果:(1)甲状腺乳头状癌、甲状腺腺瘤、结节性甲状腺肿及正常甲状腺组织平均MVD值分别为(65.54±19.21)个/HP、(54.54±11.76)个/HP、(47.85± 10.92)个/HP、(21.82±7.43)个/HP,甲状腺乳头状癌MVD值显著高于甲状腺腺瘤、结节性甲状腺肿及正常甲状腺组织(P＜0.05).甲状腺腺瘤、结节性甲状腺肿MVD值显著高于正常甲状腺组织(P＜0.05).(2)伴有淋巴结转移的甲状腺乳头状癌患者MVD值显著高于不伴有淋巴结转移者(P＜0.05)；男性甲状腺乳头状癌患者MVD值显著高于女性患者(P＜0.05)；＞40岁及≤40岁甲状腺乳头状癌患者MVD值间无统计学差异(P＞0.05).结论:甲状腺乳头状癌MVD值显著高于甲状腺腺瘤、结节性甲状腺肿及正常甲状腺组织,且伴有淋巴结转移的甲状腺乳头状癌患者MVD值显著高于不伴有淋巴结转移者.

Study on the Relationship between Microvessel Density in Benign and Malignant Thyroid Nodules

Objective： To investigate the relationship between microvessel density in benign and malignant thyroid nodules.Methods： Papillary thyroid carcinoma,thyroid adenoma,Nodular goiter and normal thyroid tissue were selected,the tissue were processed by HE and immunohistochemical SP staining.Five vascular shader intensive areas are counted under high power field（× 400）,the average MVD value =（n1 ＋ n2 ＋ n3,＋ n4,＋ n5.） / 5.Results：（1） The average MVD of papillary thyroid carcinoma,thyroid adenoma,nodular goiter and normal thyroid tissue were（65.54 ± 19.21）/HP,（54.54 ± 11.76）/HP（,47.85±10.92）/HP and（21.82 ± 7.43）/HP,the average MVD of thyroid papillary cancer was significantly higher than that of thyroid adenoma and normal thyroid tissue（P0.05）.The average MVD of thyroid adenoma and Nodular goiter were significantly higher than that of normal thyroid tissue（P0.05）.（2） The average MVD of papillary thyroid carcinoma with lymph node metastasis was significantly higher than those without lymph node metastasis（P0.05）.The average MVD of Male papillary thyroid carcinoma patients was significantly higher than female patients.（P 0.05）.There was no significant difference between 40 and ≤ 40 years of papillary thyroid carcinoma patients.Conclusion： The mean MVD value of papillary thyroid cancer was significantly higher than thyroid adenoma,nodular goiter and normal thyroid tissue,and the average MVD of papillary thyroid carcinoma with lymph node metastasis was significantly higher than those without lymph node metastasis.