低氧对间充质干细胞成骨相关基因表达的影响

目的：观察低氧对间充质干细胞（MSC）成骨相关基因表达的影响。方法：取第2代MSC，分别在常氧和3％氧浓度下培养，采用real-timeRT-PCR检测骨桥蛋白（OPN）、核心结合因子α1（Cbfal）、骨形态发生蛋白（BMP）和血管内皮生长因子（VEGF）mRNA的表达。结果：2种氧浓度下MSC的形态无明显差异，但3％氧浓度下MSC集落形成能力增强。常氧下MSC中Cbfal和BMPmRNA的表达高于3％氧浓度，差异有显著性（P〈0．05）；而OPN和VEGFmRNA的表达虽高于3％氧浓度，但差异无显著性（P〉0．05）。结论：3％氧浓度对MSC的形态没有明显影响，但增强其增殖能力，抑制其成骨分化能力。

Effect of Hypoxia on Expression of Osteogenic Related Gene of Marrow Mesenchymal Stem Cells

Objective： To observe the effect of hypoxia on expression of osteogenic related gene of marrow mesenchymal stem cells（MSC）. Methods： The second passage of the MSC was cultured in 20% 02 and 3% 02 condi- tion individually. The biological features of the cultured MSC were assessed by the real-time PCR. Results： In two oxygen concentrations, the morphology of MSC was no significant difference; but in 3% oxygen concentration, the colony formation ability of MSC increased, the difference was significant（P〈0.05）. The RT-PCR examination re- vealed that in normal oxygen condition the expression of mRNA of core binding factor cd（Cbfal） and bone mor- pho-genetic protein（BMP） in MSC was higher than those in 3% oxygen condition, the difference was significant （P〈0.05）; but not for osteopontin（OPN） and vascular endothelial growth factor（VEGF） mRNA expression（P〉 0.05）. Conclusion： In 3% oxygen condition, the survival ability of MSC was not significantly affected, but the pro- liferation ability was enhanced and the ability to differentiate into osteoblasts was inhibited.