利用表面等离子共振传感器检测苏云金芽孢杆菌cry1F蛋白

目的：建立检测苏云金芽孢杆菌（Bt）crylF蛋白的表面等离子共振（SPR）传感器方法。方法：采用SPR检测技术，利用生物分子相互作用分析原理，在金表面修饰特异性单克隆抗体，对crylF蛋白的检测进行研究。结果：该方法可以较好地检测到crylF蛋白，最低检测限可达10ng／mL，并且具有很好的特异性。结论：SPR检测方法的重复性较好，灵敏度高，目前可用于crylF蛋白的定性检测，为crylF蛋白及其他Bt蛋白的检测提供了新方法，在检测转Bt基因植物方面具有广阔的应用前景。

Using a Surface Plasmon Resonance Sensor for the Detection of Bt cry1F Protein

Objective： To establish a detection method of Bacillus thuringieasis（Bt） crylF protein by surface plasmon resonance（SPR） sensor and provide a new way for the crylF protein detection. Methods： SPR technology and biological molecules interaction analysis principle were used. Specific monoclonal antibody was modified on the gold surface for the research of crylF protein detection. Results： The method can detect crylF protein very well with the limit of detection 10 ng/mL and has a good specificity. Conclusion： The SPR detection mehod has a sufficient repeatablility and high sensitivity. It can be used for the qualitative detection of crylF protein and pro- vides a new way for the detection of crylF protein as well as other Bt proteins. Thus it will be widely applied in the detection of Bt gene-modified plants in the future.