Bacillus thuringiensis fluorescence induced by anaerobiosis and its relation to the cellular functional state

The fluorescence spectra of Bacillus thuringiensis native cells at 340 nm induction have been investigated. The spectral curve has 440-445 and 510-515 nm maximums of fluorescence of pyridine nucleotides (PN) and flavoproteins (FP). The induction of PN and FP fluorescence under anaerobiosis resulted from bacterium breath has been found. The fluorescence induction is connected with the viability of cells. The amplitude of induced fluorescence is in a linear dependence on the number of vegetative cells in medium. The given experimental approaches may be used in a course of development of methods of continuous control over the physiological state change of microorganisms.