A retinoic acid responsive gene, MK, produces a secreted protein with heparin binding activity |
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| Authors: | M Tomomura K Kadomatsu M Nakamoto H Muramatsu H Kondoh K Imagawa T Muramatsu |
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| Affiliation: | Department of Biochemistry, Faculty of Medicine, Kagoshima University, Japan. |
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| Abstract: | MK is a gene whose expression increases transiently during retinoic acid-induced differentiation of embryonal carcinoma cells. MK polypeptide was secreted by differentiating HM-1 embryonal carcinoma cells and by L-cells transfected with an MK cDNA under the control of the beta-actin promoter and Rous sarcoma virus enhancer. MK polypeptide was found to have heparin binding activity. Conditioned medium of the transfected L-cells promoted growth of PC-12 pheochromocytoma cells. These findings support the view that MK polypeptide is a secreted factor involved in regulation of growth and differentiation. |
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