Identification of a novel DNA element that promotes cell-to-cell transformation in Escherichia coli |
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Authors: | Sobue Rina Kurono Naomi Etchuya Rika Maeda Sumio |
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Institution: | Graduate School of Humanities and Sciences, Nara Women's University, Kitauoya-Nishimachi, Nara, Japan. |
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Abstract: | Recently, we discovered a novel phenomenon, "cell-to-cell transformation" by which non-conjugative plasmids are transmitted horizontally in co-cultures of Escherichia coli F(-) strains. In this study, we aimed to identify the DNA element responsible for the high cell-to-cell transformability of pHSG299. By transplanting pHSG299 DNA fragments into pHSG399, a plasmid showing low transformability, we discovered that a specific 88 bp fragment of pHSG299 significantly promoted pHSG399 transformability. Although several short motif-like repetitive sequences (6-10 bp) were present in the 88 bp sequence, no known DNA motifs were recognized, suggesting that this 88 bp sequence (cell-to-cell transformation promoting sequence, CTPS; Accession number: AB634455) is a novel DNA element. |
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