Eight new polymorphic microsatellite DNA markers for Sakhalin taimen Parahucho perryi |
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Authors: | Theodora Kopun Kathrin A Winkler Steven Weiss |
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Institution: | 1. Institute of Zoology, Universit?tsplatz 2, 8010, Graz, Austria
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Abstract: | We developed eight polymorphic microsatellite markers for the endangered salmonid Parahucho perryi from genomic libraries enriched for (GACA)n and (GATA)n repeat sequences. Emphasis was placed on developing highly polymorphic,
perfect repeats that could be scored with confidence. Six tetra- and two di-nucleotide loci were screened in 49 individuals
from two populations on Sakhalin Island, Russia. Allelic variation was high with eight to 23 alleles per locus and expected
heterozygosity ranging from 0.48 to 0.93. These highly variable markers should prove useful in evaluating inbreeding, gene
flow and population structure in Sakhalin taimen throughout its range. |
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Keywords: | Salmonidae Sakhalin Island Tetra-nucleotide repeats Endangered species |
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