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Synthesis of peptide-modified pyoverdins by a fluorescent Pseudomonas strain grown in isoleucine-supplemented medium |
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| Authors: | Ongena Marc Jacques Philippe de Pauw Edwin Thonart Philippe |
| Institution: | (1) Centre Wallon de Biologie Industrielle, Unité de Bioindustries, Faculté Universitaire des Sciences Agronomiques, B-5030 Gembloux, Belgium;(2) Laboratoire de Spectrométrie de Masse, Université de Liège, Batiment B6, B-4000 Liège/Sart-Tilman, Belgium;(3) Present address: Centre Wallon de Biologie Industrielle, Université de Liège, Batiment 40, B-4000 Liège/Sart Tilman, Belgium |
| Abstract: | Summary The pattern of pyoverdin-type siderophores produced byPseudomonas putida BTP16 in iron-limited succinate medium supplemented or not with isoleucine was characterized by electrospray ionization mass spectrometry. Two new compounds were observed in culture supernatants from BTP16 grown in the presence of isoleucine. For both molecules, mass differences with pyoverdins originally synthesized by the strain were only interpreted considering the incorporation of Ile into the peptide chain. Further evidence supporting this phenomenon was provided by radioactivity incorporation in pyoverdins produced in the presence of14C-labeled Ile. Our results are discussed in relation to the non-ribosomal mechanism supposed to be involved in the synthesis of these molecules. |
| Keywords: | electrospray ionization mass spectrometry iron acquisition non-ribosomal peptide synthetase Pseudomonas putida siderophore |
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