A Bacillus subtilis dnaG mutant harbours a mutation in a gene homologous to the dnaN gene of Escherichia coli |
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| Authors: | N Ogasawara S Moriya G Mazza H Yoshikawa |
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| Affiliation: | 1. Department of Genetics,The University of Osaka, Medical School, 3-57, Nakanoshima 4-chome, Kitaku, Osaka 530 Japan Tel. 06-443-5531;2. Dipartimento di Genetica e Microbiologia ''A. Buzzati-Traverso'', Universitá di Pavia, via S. Epifanio, 14-27100 PaviaItaly Tel. (0382) 31036;1. School of Biological and Chemical Sciences, Queen Mary University of London, London E1 4NS, UK;2. Research Center for Engineering Ecology and Nonlinear Science, North China Electric Power University, Beijing 102206, China;1. Crop Science Laboratory, Graduate School of Agriculture, Kyoto University, Kyoto-city, Kyoto 606-8502, Japan;2. Research Fellow of Japan Society for the Promotion of Science, Japan;3. Soybean and Field Crop Applied Genomics Research Unit, Institute of Crop Science, National Agriculture and Food Research Organization (NARO), Tsukuba-city, Ibaraki, Japan;4. JST, PRESTO, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan;5. Division of Basic Research, Institute of Crop Science, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki 305-8518, Japan;1. Universitat de Girona, Av. Lluis Santalo, S/N Campus Montilivi, Edifici P-4, E-17071 Girona, Spain;2. Newronia, Parc Científic i Tecnològic, Edifici Narcís Monturiol, c/Emili Grahit, 91, E-17003 Girona, Spain;3. School of Molecular Sciences, Arizona State University, Tempe, Arizona 85287;4. Center for Bioenergy and Photosynthesis, Arizona State University, Tempe, Arizona 85287;5. Biodesign Center for Applied Structural Discovery, Arizona State University, Tempe, Arizona 85287;6. Biodesign Center for Single Molecule Biophysics, Arizona State University, Tempe, Arizona 85287 |
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| Abstract: | A dnaG mutation of Bacillus subtilis, dnaG5, was found to be linked closely to recF. We have reported previously that two putative dna genes, 'dnaA' and 'dnaN', highly homologous to Escherichia coli's dnaA and dnaN, respectively, were located adjacent to recF [Ogasawara et al., EMBO J., 4 (1985) 3345-3350]. Transformation by various fragments cloned from the 'dnaA'-recF region of the wild-type cell revealed that a 532-bp AluI fragment containing 5'-portion of the 'dnaN' gene could transform the dnaG5 mutation. The nucleotide (nt) sequence of the same fragment cloned from the mutant cell shows a single nt change in the ORF of 'dnaN' which in turn causes a single amino acid alteration from Gly to Arg. The 'dnaN' gene is now proven to be a dna gene, mutations in which result in instant arrest of chromosomal replication. |
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