Identification and expression analysis of miRNAs from nitrogen-fixing soybean nodules |
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| Authors: | Yanwei Wang Pingchuan Li Xiaofeng Cao Xiujie Wang Aimin Zhang Xia Li |
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| Affiliation: | 1. The State Key Laboratory of Plant Cell & Chromosome Engineering, Center of Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 286 Huaizhong Road, Shijiazhuang, Hebei 050021, PR China;2. Graduate University, Chinese Academy of Sciences, Beijing 100039, PR China;3. The State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;1. Department of Crop Science and Biotechnology, Dankook University, San 29 Anseodong, Cheonan, Chungnam 330-714, Republic of Korea;2. Department of Life Science (BK21 Program), Chung-Ang University, Seoul 157-756, Republic of Korea;1. School of Biological Sciences, Washington State University, Pullman, WA 99164, USA;1. Takasaki Advanced Radiation Research Institute, National Institutes for Quantum and Radiological Science and Technology (QST), Takasaki, Gunma, 370-1292, Japan;2. Japan Society for the Promotion of Science, Japan |
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| Abstract: | miRNAs are ～21 nt non-coding RNAs and play important roles in plant development and response to stress. Symbiotic nitrogen fixation (SNF) is agronomically important for reducing the need of nitrogen fertilizers. The soybean root nodule is the place where SNF takes place. To identify miRNAs that are possibly involved in nitrogen fixation in soybean functional nitrogen-fixing nodules, a small library of RNAs was constructed from the functional nodules harvested 28 days after inoculation with rhizobium. Thirty-two small RNA sequences were identified as belonging to 11 miRNA families. Eight miRNAs are conserved across plant species, twenty are specific to soybean, and the four remaining miRNAs are novel. Expression analysis revealed that miRNAs were differentially expressed in the different tissues. Combinatorial miRNA target prediction identified genes that are involved in multiple biological processes. The results suggest that miRNAs play critical and diverse roles in SNF, nutrient acquisition, and plant development. |
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