A neuromedin-pyrokinin-like neuropeptide signaling system in Caenorhabditis elegans |
| |
| Authors: | Marleen Lindemans Tom Janssen Steven J. Husson Ellen Meelkop Liesbet Temmerman Elke Clynen Inge Mertens Liliane Schoofs |
| |
| Affiliation: | 3. Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan;4. the Molecular Entomology Laboratory, RIKEN Advanced Science Institute, Wako, Saitama 351-0198, Japan;5. the United States Department of Agriculture-Arid Land Agricultural Research Center, Maricopa, Arizona 85138;1. United States Department of Agriculture–Agricultural Research Service, Center for Medical, Agricultural and Veterinary Entomology (CMAVE), 1600 SW 23rd Drive, Gainesville, FL 32608, USA;2. Navy Entomology Center of Excellence, Box 43, Naval Air Station, Jacksonville, FL 32212-0043, USA;1. Department of Biology, Institute for Zoology, Functional Peptidomics Group, University of Cologne, D-50674 Cologne, Germany;2. Department of Entomology, The Volcani Center, Bet Dagan 50250, Israel;1. USDA-ARS Horticultural Crops Research Unit,3420 NW Orchard Avenue, Corvallis, OR, 97330, USA;2. Department of Crop and Soil Science, Oregon State University, Corvallis, OR, 97331, USA;3. USDA-ARS Forage Seed and Cereal Research Unit, 3450 SW Campus Way, Corvallis, OR, 97331, USA;1. Department of Entomology, Kansas State University, Manhattan, KS 66506, United States;2. Areawide Pest Management Research, Southern Plains Agricultural Research Center, USDA, 2881 F/B Road, College Station, TX 77845, United States |
| |
| Abstract: | Neuromedin U (NMU) in vertebrates is a structurally highly conserved neuropeptide of which highest levels are found in the pituitary and gastrointestinal tract. In Drosophila, two neuropeptide genes encoding pyrokinins (PKs), capability (capa) and hugin, are possible insect homologs of vertebrate NMU. Here, the ligand for an orphan G protein-coupled receptor in the nematode Caenorhabditis elegans (Ce-PK-R) was found using a bioinformatics approach. After cloning and expressing Ce-PK-R in HEK293T cells, we found that it was activated by a neuropeptide from the C. elegans NLP-44 precursor (EC50 = 18 nM). This neuropeptide precursor is reminiscent of insect CAPA precursors since it encodes a PK-like peptide and two periviscerokinin-like peptides (PVKs). Analogous to CAPA peptides in insects and NMUs in vertebrates, whole mount immunostaining in C. elegans revealed that the CAPA precursor is expressed in the nervous system. The present data also suggest that the ancestral CAPA precursor was already present in the common ancestor of Protostomians and Deuterostomians and that it might have been duplicated into CAPA and HUGIN in insects. In vertebrates, NMU is the putative homolog of a protostomian CAPA-PK. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
