Evidence of free fatty acid interdigitation in stratum corneum model membranes based on ceramide [AP] by deuterium labelling |
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| Authors: | Annett Schroeter Mikhail A Kiselev Thomas Hauß Silva Dante Reinhard HH Neubert |
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| Institution: | 1. Martin-Luther-Universität Halle-Wittenberg, Institute of Pharmacy, Department of Pharmaceutics and Biopharmaceutics, Halle (Saale), Germany;2. Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research, Dubna, Moscow Reg., Russia;3. Helmholtz-Zentrum Berlin für Materialien und Energie, Berlin, Germany;4. Technische Universität Darmstadt, Dept. of Chemistry, Physical Biochemistry, Darmstadt, Germany |
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| Abstract: | This research paper provides direct evidence concerning the localisation of free fatty acids in stratum corneum lipid model membranes. We employed partially deuterated free fatty acids to gain further information about the assembly of a stratum corneum lipid model membrane based on a ceramide of the phytosphingosine-type (ceramide AP]) with particular respect to the position of the deuterated groups of the free fatty acids. The application of behenic-22,22,22-d3-acid and cerotic-12,12,13,13-d4-acid confirmed that the short-chain ceramide AP] forces the longer-chained free fatty acids to incorporate into the bilayer created by ceramide AP]. The ceramide AP] molecules determine the structural assembly of this model membrane and obligate the long-chain free fatty acids to either arrange inside this formation or to separate as a fatty acid rich phase. |
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