Demonstration and Partial Characterization of a Bacterial Growth Enhancer in sera |
| |
Authors: | Kanna Okayama Takeshi Honda Shigeaki Matsuda Tadashi Saito Masaya Kawase |
| |
Institution: | (1) Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan;(2) College of Nursing Art and Science, University of Hyogo, 13-71 Kitaoji-cho, Akashi Hyogo, 673-8588, Japan;(3) Laboratory of Genomic Research on Pathogenic Bacteria, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan;(4) Radioisotope Research Center, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka 560-0043, Japan;(5) Faculty of Bioscience, Nagahama Institute of Bio-science and Technology, 1266 Tamaura-cho, Nagahama, Shiga 526-0829, Japan |
| |
Abstract: | During our research into the pathogenesis of Vibrio parahaemolyticus, we noticed that the concentration of serum added to the tissue culture medium (Dulbecco’s modified Eagle’s medium: DMEM)
greatly affected its growth. Using gel filtration column chromatography, we clearly demonstrated that serum contains not only
a bacterial growth inhibitor (BGI) but also a bacterial growth enhancer (BGE) for Vibrio parahaemolyticus. Our data indicate that the BGI is transferrin, whereas the BGE seems to be an undescribed small molecule (molecular weight
of 1,000–3,000 Da) and is associated with magnesium and molybdenum ions. BGE activity was not decreased by heat treatment
(at 60 or 100°C for 30 min) and affected the growth rate of a wide range of Gram-negative and Gram-positive bacteria. The
addition of EDTA into DMEM lowered the growth rate, whereas the addition of BGE restored the growth activity. This study suggests
that sera contain a previously undescribed small BGE molecule. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|