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Strategies for covalent attachment of DNA to beads
Authors:Steinberg Gali  Stromsborg Katie  Thomas Lynette  Barker David  Zhao Chanfeng
Institution:Illumina, Inc., 9885 Towne Centre Drive, San Diego, CA 92121, USA.
Abstract:Several covalent attachment chemistries were tested for the immobilization of DNA onto glass beads. The comparison was based on the ability of these chemistries to produce derivatized beads that give good hybridization signals. Cyanuric chloride, isothiocyanate, nitrophenyl chloroformate, and hydrazone chemistries gave us the best (yet comparable) hybridization signals. We further characterized the cyanuric chloride method for the number of attachment sites, number of hybridizable sites, hybridization kinetics, effect of linker length on hybridization intensity and stability of the derivatized beads.
Keywords:covalent attachment  immobilization  DNA: glass beads  hybridization
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