NADPH:cytochrome P-450(c) reductase: Biochemical characterization in rat brain and cultured neurons and evolution of activity during development |
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Authors: | J. F. Ghersi-Egea A. Minn J. L. Daval Z. Jayyosi V. Arnould H. Souhaili-El Amri G. Siest |
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Affiliation: | (1) Centre du Médicament, CNRS URA 597, Université de Nancy-1, 30 rue Lionnois, 54000 Nancy, France;(2) INSERM U 272, 30 rue Lionnois, 54000 Nancy, France |
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Abstract: | NADPH:cytochrome P-450 (c) reductase is a microsomal enzyme which is involved in the cytochrome P-450-dependent biotransformation of many exogenous agents as well as of some endogenous molecules. Using cytochromec as a substrate, the kinetic parameters of this enzyme were determined in brain microsomes. The comparison of the NADPH:cytochrome P-450 reductase's Vmax values and cytochrome P-450 contents in both fractions, suggests a role of cerebral NADPH:cytochrome P-450 reductase in cytochrome P-450 independent pathways. This is also supported by the different developmental pattern of brain enzyme as compared to the liver enzyme, and by the presence of a relatively high NADPH:cytochrome P-450 reductase activity in immature rat brain and neuronal cultures, while cytochrome P-450 was hardly detectable in these preparations. The enzyme activity was not induced by a phenobarbital chronic treatment neither in the adult brain nor in cultured neurons, suggesting a different regulation of the brain enzyme expression. |
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Keywords: | NADPH-Cytochrome P-450 reductase Cytochrome P-450 neuronal culture development induction |
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