Analysis of rat brain microdialysate by gas chromatography—high-resolution selected-ion monitoring mass spectrometry |
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Authors: | S.Y. Chang T.A. Moore L.L. Devaud L.C.E. Taylor E.B. Hollingsworth |
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Affiliation: | S.Y. Chang, T.A. Moore, L.L. Devaud, L.C.E. Taylor*,E.B. Hollingsworth |
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Abstract: | Gas chromatography—high-resolution selected-ion monitoring mass spectrometry was used to analyze catecholamine metabolites in rat brain microdialysate. Dialysate samples were collected in vials containing stable isotope analogues of homovanillic acid (HVA), 3-methoxy-4-hydroxyphenylglycol (MHPG) and 5-hydroxyindoleacetic acid (5HIAA) and analyzed as their trimethylsilyl derivatives. The metabolite levels were monitored at 20-min intervals throughout the time course of the experiment, beginning immediately after surgery and implantation of the dialysis probe and ending 4 h after amphetamine treatment. The levels of HVA were observed to decrease after amphetamine treatment, while those of MHPG and 5HIAA did not change significantly. |
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