PD1, an S-like RNase gene from a self-incompatible cultivar of almond |
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Authors: | I. Van Nerum A. C. Certal M. M. Oliveira J. Keulemans W. Broothaerts |
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Affiliation: | (1) Fruitteeltcentrum K.U.Leuven, W. de Croylaan 42, 3001 Heverlee, Belgium e-mail: ilse.nerum@agr.kuleuven.ac.be Fax: +32–16–322966, BE;(2) Dept. Biologia Vegetal, Campo Grande, 1700 Lisbon, Portugal, PT;(3) IBET, Quinta do Marquês, 2780 Oeiras, Portugal, PT |
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Abstract: | Many flowering plants contain stylar S-RNases that are involved in self-incompatibility and S-like RNases of which the biological function is uncertain. This paper reports the deduced amino acid sequence of an S-like RNase gene (PD1) from the self-incompatible plant Prunus dulcis (almond). The amino acid sequence of PD1, which was derived from cDNA and genomic DNA clones, showed 34–86% identity to acidic plant S-like RNases reported so far, with the highest degree of similarity being to an S-like RNase from Japanese pear (Pyrus pyrifolia). Based on RNA hybridisation experiments it appears that, like for many other S-like RNases, the expression of PD1 is not pistil-specific. Analysis of the genomic structure revealed the presence of three introns, of which one is similar in location to that of the related S-RNase gene from Solanaceae and Rosaceae. At least four bands hybridising to PD1 were found upon Southern hybridisation, suggesting the presence of a multigene family of S-like RNase genes in almond. The putative biological function of PD1 is discussed. Received: 22 November 1999 / Revision received: 18 February 2000 · Accepted: 13 March 2000 |
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Keywords: | Almond cDNA cloning Prunus dulcis Sequence comparison S-like RNase |
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