| 中国红豆杉细胞紫杉醇合成期与非紫杉醇合成期基因表达差异初步分析 |
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| 引用本文： | 胡国斌 梅兴国 龚伟 柯铁. 中国红豆杉细胞紫杉醇合成期与非紫杉醇合成期基因表达差异初步分析[J]. 生物工程学报, 2002, 18(4): 512-515 |
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| 作者姓名： | 胡国斌 梅兴国 龚伟 柯铁 |
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| 作者单位： | 1. 华中科技大学生命科学与技术学院,武汉,430074
2. 军事医学科学院毒物药物研究所,北京,100850 |
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| 基金项目： | 国家“九五”科技攻关重点资助项目 (No .96 C0 2 0 3 0 1)~~ |
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| 摘 要： | 紫杉醇 (Ｐａｃｌｉｔａｘｅｌ,商品名为Ｔａｘｏｌ)是存在于红豆杉属(Ｔａｘｕｓｓｐ .)植物中的一种四环二萜酰胺类化合物 ,因其具有广谱强效的抗癌活性和独特的抗癌机理而倍受人们关注。然而 ,紫杉醇天然资源十分有限 ,自 1992年美国食品与药物管理局 (ＦＤＡ)批准紫杉醇用于癌症的临床治疗以来 ,其供需矛盾日益尖锐[1 ] 。目前 ,普遍认为采用红豆杉细胞悬浮培养生产紫杉醇是解决其药源紧缺问题的最佳途径之一。迄今为止 ,在红豆杉细胞的代谢规律和培养方面均取得一定成果 ,但前景仍不容乐观 ,离工业化大规模生产还有相当大的距离 ,主要…
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| 关 键 词： | mRNA差异显示， 基因表达， 紫杉醇生物合成 |
| 文章编号： | 1000-3061(2002)04-0512-04 |
| 修稿时间： | 2001-12-21 |
Differences in Gene Expression Between Taxus chinensis Cells During Taxol-synthesis Phase and Those During Non- Taxol- synthesis Phase |
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| HU Guo-Bin MEI Xing-Guo GONG Wei KE Tie. Differences in Gene Expression Between Taxus chinensis Cells During Taxol-synthesis Phase and Those During Non- Taxol- synthesis Phase[J]. Chinese journal of biotechnology, 2002, 18(4): 512-515 |
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| Authors: | HU Guo-Bin MEI Xing-Guo GONG Wei KE Tie |
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| Affiliation: | School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China. hugbonline@263.net |
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| Abstract: | In plant, evocation of secondary metabolism is associated with complex biochemical and molecular events that are regulated by developmental and environmental factors. In order to get more information about Taxol biosynthesis, comparison of mRNA populations from Taxus chinensis cells during Taxol-synthesis phase and those during non-Taxol-synthesis phase were performed by mRNA differential display. The results suggested that genes specifically expressed in the Taxol-synthesis phase might be involved in Taxol biosynthesis. |
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| Keywords: | gene expression mRNA differential display Taxol biosynthesis |
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