软枣猕猴桃试管苗叶片和茎段的愈伤组织诱导及植株再生

从软枣猕猴桃试管苗茎段和叶片诱导出愈伤组织并得到再生植株。茎段外植体容易愈伤化，但其愈伤组织难以分化，叶片外植体不易愈伤化，但其愈伤组织容易分化。ＭＳ培养基分别附加ＢＡＰ、Ｋｉｎ、ＴＤＺ或ＣＰＰＵ都不能诱导芽的分化，而ＭＳ附加玉米素能有效地诱导芽分化，其中以２．０ｍｇ／Ｌ玉米素效果最好。

CALLUS INDUCTION AND PLANT REGENERATION FROM LEAVES AND STEM SEGMENTS OF IN VITRO SHOOTS OF ACTINIDIA ARGUTA

Calli were induced and whole plants were regenerated from in vitro culture of leaf and stem segments of Actinidia arguta (Sieb. et Zucc. )Planch. ex Miq.. Leaf explants were difficult to induce callus but the calli were easy to differentiate buds;on the contrary,stem segments were easy to callusise but hard to obtain bud regeneration. Bud regeneration was not obtained on MS medium supplemented with BAP (0. 5, 1. 0 and 2.0mg/L),Kin (0. 5, 1. 0 and 2. 0mg/L),TDZ (0. 0001, 0. 01 and 1. 0mg/L),and CPPU (0. 00025, 0. 025 and 2. 5mg/L, in the presence of 0. 1mg/L IAA), respectively. In contrast,MS medium added with zeatin (0. 5, 1. 0, 2. 0 and 3. 0mg/L) was found to be proper for bud differentiation, and the best result was obtained from MS medium with 2. 0mg/L zeatin.