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Immobilization of Candida rugosa lipase in lyotropic liquid crystals and some properties of the immobilized enzyme
Authors:Dieter Frense  Ulrich Lange  Winfried Hartmeier
Affiliation:(1) Department of Biotechnology, Aachen University of Technology, D-52056 Aachen, Germany
Abstract:Summary Lipase from Candida rugosa has been immobilized in lyotropic liquid crystals consisting of a nonionic surfactant, hexane, and aqueous buffer with the enzyme. The kinetics of butyl butyrate synthesis, diffusion effects, and enzyme stability were investigated. Some basic rules have been formulated for a rational medium design in liquid-crystalline matrices.
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