Golgi Impregnation After Formalin Fixation |
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Authors: | Vandee Vaisamruat Arthur Hess |
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Institution: |
a Fellow of the Thai Government in Anatomy, Washington University School of Medicine,
b Siriraj Hospital, University of Medical Sciences, Dhonburi, Thailand
c Department of Anatomy, Washington University School of Medicine, St Louis, Missouri |
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Abstract: | Formalin fixed (10% aqueous) brain from cat, rabbit and man cut to blocks 3-4 mm. thick was placed in a mixture of potassium bichromate, 5 g.; chloral hydrate, 3 g. and water 90 ml. for 24 hours. The specimens were rinsed through 3 changes of water, and transferred through 3 changes of 1% silver nitrate, 1-3 minutes each, then placed for 24 hours in 1.5% silver nitrate. Frozen sections, 40-50 μ were dehydrated and mounted with a cover glass, using Permount. No deterioration of the stain was seen after 5 months. Some brains had been in formalin for 9 months; others only 7 days. |
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Keywords: | |
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