Hydrogen-bonding effects and 13C-NMR of the DNA double helix |
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| Authors: | P N Borer S R LaPlante N Zanatta G C Levy |
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| Affiliation: | NIH Research Resource, Syracuse University, NY 13244-1200. |
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| Abstract: | 13C-nmr chemical shifts of the nucleotides in DNA are sensitive to hydrogen bonding, especially for three of the carbons immediately bonded to exocyclic oxygen or nitrogen atoms acting as H-bond acceptors or donors. GuoC2, GuoC6 and ThdC4 are strongly deshielded (about 1 ppm) upon Watson-Crick pairing in oligodeoxynucleotide duplexes, regardless of the base sequence. Deshielding at these sites may be useful to distinguish bases involved in Watson-Crick pairs from unpaired bases. |
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