Solvent Exchange Rates of Side-chain Amide Protons in Proteins |
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Authors: | Ponni Rajagopal Bryan E Jones Rachel E Klevit |
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Institution: | (1) Department of Biochemistry, University of Washington, Biomolecular Structure Center, Box 357742, Seattle, WA, 98195-7742, U.S.A |
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Abstract: | Solvent exchange rates and temperature coefficients for Asn/Gln side-chain amide protons have been measured in Escherichia coli HPr. The protons of the eight side-chain amide groups (two Asn and six Gln) exhibit varying exchange rates which are slower than some of the fast exchanging backbone amide protons. Differences in exchange rates of the E and Z protons of the same side-chain amide group are obtained by measuring exchange rates at pH values > 8. An NOE between a side-chain amide proton and a bound water molecule was also observed. |
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Keywords: | HPr side-chain amide proton solvent exchange rates temperature coefficients |
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