Involvement of nucleophosmin/B23 in the cellular response to curcumin |
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Authors: | Chien-Hui WengBenjamin Yat-Ming Yung Jing-Jei WengMing-Hsiu Wu |
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Institution: | a Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung 824, Taiwanb Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kongc Institute of Biopharmaceutical Science, National Yang-Ming University, Taipei 112, Taiwand Department of Nutrition and Health Science, Fooyin University, 151 Chin-hsueh Road, Ta-liao Hsiang, Kao-hsiung Hsien 831, Taiwan, Republic of China |
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Abstract: | Nucleophosmin (NPM/B23) is a nucleolar phosphoprotein involved in cellular response to many different stimuli. Herein, we studied the molecular mechanism of NPM/B23 induction by curcumin, a natural AP-1 inhibitor with antitumor properties. Exposure to 5-30 μM curcumin significantly and dose-dependently increased the level of NPM/B23 in non-transformed NIH 3T3 cells but not HeLa cells and F9 cells. Besides, the transformed F9 and HeLa cells are more sensitive to curcumin-induced cell death and growth inhibition than NIH 3T3 cells. Overexpression of c-Jun, but not c-Fos, decreased ∼40% of NPM/B23 and enhanced the sensitivity of NIH 3T3 cells to 30 μM curcumin. Furthermore, down-regulation of NPM/B23 by transfection with NPM/B23 antisense plasmid enhanced the sensitivity to curcumin-induced cell death and growth inhibition. These results indicated that NPM/B23 expression regulates cellular sensitivity to curcumin. Besides, NPM/B23 knockdown may facilitate as a novel strategy to promote the sensitivity of cancer cells to curcumin. |
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Keywords: | Curcumin Nucleophosmin/B23 Activator protein-1 c-Jun c-Fos |
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