Valine-resistant plants from in vitro selected tobacco cells |
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Authors: | Jean-Pierre Bourgin |
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Institution: | (1) Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, F-78000 Versailles, France |
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Abstract: | Summary Growth inhibition of protoplasts and cells of tobacco (Nicotiana tabacum L.) occurs when 0.1 to 10 mM L-valine is present in the culture medium but not if L-isoleucine is simultaneously added. Mesophyll protoplasts from haploid tobacco were submitted to ultraviolet irradiation and cell colonies derived from these protoplasts were plated in a medium containing valine. Plants were regenerated from five presumptive resistant calli and retested for valine-resistance. Among the recovered plants two were definitely resistant to valine. This trait was transmitted in sexual crosses as a mendelian character. |
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