Hypotonic treatment leads to chromosomal aberrations but not to sister-chromatid exchanges in human lymphocytes |
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Authors: | S Kalweit C Nowak G Obe |
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Institution: | Freie Universit?t Berlin, Institut für Genetik, Germany. |
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Abstract: | Human peripheral lymphocytes were isolated from whole blood and exposed to culture medium of reduced osmolality. This hypotonic treatment led to a significant increase in the frequencies of chromosomal aberrations when the osmolality was reduced to 60 mOsm/kg H2O and below. Maximum damage occurred when the hypotonic treatment was done 27 or 30 h after starting the cultures. We also looked for the induction of sister-chromatid exchanges (SCE) by hypotonic culture conditions, but the SCE frequencies were not influenced. |
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