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Alkane hydroxylase homologues in Gram-positive strains |
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| Authors: | van Beilen Jan B Smits Theo H M Whyte Lyle G Schorcht Susanne Röthlisberger Martina Plaggemeier Thorsten Engesser Karl-Heinrich Witholt Bernard |
| Institution: | Institute of Biotechnology, Swiss Federal Institute of Technology (ETH), ETH-Hönggerberg, CH-8093 Zürich, Switzerland.;ENAC/ISTE/LBE, EPFL, Lausanne, Switzerland.;NRC-Biotechnology Research Institute, Montreal, Quebec, Canada.;GeneScan Analytics, Fahrenheitsstraße 1, Bremen, Germany.;Institut für Siedlungswasserbau, Abt. Biologische Abluftreinigung, Bandtäle 2, Stuttgart, D-70569, Germany. |
| Abstract: | We isolated Gram-positive alkane-degraders from soil and a tricking-bed reactor, and show using polymerase chain reaction (PCR) with degenerate alkane hydroxylase primers and Southern blots that most Rhodococcus isolates contain three to five quite divergent homologues of the Pseudomonas putida GPo1 alkB gene. Two Mycobacterium isolates each contain one homologue, however there is no evidence for the presence of alkB homologues in the remaining strains. |
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