Efficient synthesis of a sialyl T-antigen-linked glycopeptide by the chemoenzymatic method |
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Authors: | Ajisaka K Miyasato M |
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Affiliation: | Meiji Institute of Health Science, Meiji Milk Products Co. Ltd, Odawara, Japan. kajisaka@mbc.sphere.ne.jp |
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Abstract: | A sialyl T-antigen-linked tetrapeptide was prepared by the combined method of chemical synthesis and enzymatic synthesis. The GalNAc-linked peptide was first obtained by using a commercial peptide synthesizer, and then a galactose residue was attached with beta-(1-->3)-linkage by transglycosylating with a recombinant beta-galactosidase from Bacillus circulans. The sialic acid residue was then combined by alpha-(2-->3)-linkage with sialytransferase from rat liver. |
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