精原干细胞移植受体鼠模型的建立

目的 通过对4周龄昆明白小鼠腹腔内单次注射白消安来制作精原干细胞移植受体鼠模型。方法 将实验动物分为4组,A、B、C组注射白消安的剂量分别是30 mg/kg4、0 mg/kg5、0 mg/kg体重,D组为对照组。注射后每天记录小鼠的存活情况,注射白消安后的20 d3、0 d4、0 d称量睾丸重量、测定血常规、制作并观察睾丸组织学切片、统计曲细精管的中空率。结果 A、B、C、D组的死亡率分别是25.00%3、1.58%、80.00%、0.00%,注射白消安后30 d各组小鼠曲细精管中空率分别是45.25%、75.25%、1.50%、0.00%,白细胞、红细胞、血小板数量等血常规指标均恢复正常。结论 腹腔内单次注射30 mg/kg或40 mg/kg剂量的白消安,死亡率较低（25.00%、31.58%）,30 d后曲细精管中空率较高（45.25%7、5.25%）、血常规指标恢复正常,适合做精原干细胞移植受体。

Model Establishment of Transplantation of Spermatogonial Stem Cells in Mice

Objective To obtain an optimized treatment system for transplantation of spermatogonial stem cells. Method Different doses （30 mg/kg,40 mg/kg,50 mg/kg,0 mg/kg） of bulsulfan were injected into four groups of mice separatively. Survival rate was recorded every day after injection, testis weight, routine analysis of blood, sections observation statistics were conducted 20, 30, 40 days later. Result The mortality of group A, B,C and D were 25.00% ,31.58% ,80.00% ,0.00%, separately. Hollow rate were 45.25% ,75.25% ,1.50% ,0.00% 30 days after busulfan injection. Conclusion Group A and B had lower mortality （25.00%, 31,58% ） and higher hollow rate （45.25%, 75.25% ） when 30 mg/kg or40 mg/kg busulfan were injected. Because WBC, RBC, PLT were normal at these two doses, these treatment of spermatogonial stem cell recipient mice is acceptable.