| 外源性神经生长因子对痴呆老龄鼠学习和记忆能力影响的初步探讨 |
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| 引用本文： | 王丽萍,;邱泽文,;梁桂英,;王靖宇,;姜春玲,;姜长斌. 外源性神经生长因子对痴呆老龄鼠学习和记忆能力影响的初步探讨[J]. 中国实验动物学杂志, 2007, 0(2): 88-91 |
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| 作者姓名： | 王丽萍, 邱泽文, 梁桂英, 王靖宇, 姜春玲, 姜长斌 |
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| 作者单位： | [1]大连大学中山医院,大连116000; [2]大连医科大学实验动物中心,大连116027; [3]长春师范学院,长春130032; [4]大连医科大学第二临床医院,大连116027 |
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| 基金项目： | [基金项目]大连市科技攻关计划（2006E11SFU78）. |
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| 摘 要： | 目的探讨外源性神经生长因子（NGF）对痴呆老龄鼠智能影响的机制。方法本实验选用18月龄ICR小鼠45只，应用整体实验和免疫组织化学技术，观察给药前后各组迷宫试验、胆碱乙酰转移酶活性（chAT-IR）、神经生长因子抗体（Anti-NGF）在相关脑区的表达并进行统计学分析。结果用药后学习及记忆能力有明显改善（P〈0．05），与用药前比较ChAT-IR明显增加（P〈0．01），NGF抗体表达明显增加（P〈0．01）。结论外源性NGF对痴呆老龄鼠的智能有改善作用；能够使相关脑区ChAT-IR增加；相关脑区Anti-NGF表达增加；外源性NGF能够保护、支持、修复神经原。
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| 关 键 词： | 神经生长因子(NGF) 痴呆 胆碱乙酰转移酶(chAT) 老龄小鼠 |
Effects of Exogenous NGF on Memory Ability in Demential Senile Mice |
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| Affiliation: | Wang Li-ping , Qiu Ze-wen , Liang Gui-ying , Wang Jing-yu ,Jiang Chun-ling , Jiang Chang-bin (1. Affiliated Zhongshan Hospital of the Dalian University, Dalian 116000, China; 2. Laboratory Animal Center of the Dalian Medical University, Dalian 116027, China; 3. Changchun Normal University, Changchun 130032, China; 4. The Second Affiliated Hospital of Dalian Medical University, Dalian 116027, China) |
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| Abstract: | Objective To explore the effect and mechanism of action of NGF on the intelligence in demential senile mice. Method Fourty five 18 month-old ICR mlee were selected and randomly divided into two groups. The expression of choline acetyltransterase （ChAT-IR） and nerve growth factor （NGF） in various areas of the brain was assyed. Mirro-labyrinth experiment was eondueted before and after administration of exogenous NGF. ChAT-IR aetivity and Anti-NGF immunohistochemical examination were performed. Result The inteiligence of senile miee were changed. Signifieant changes of ChAT-IR and NGF expression in areas in the brain and in Mirro labyrinthian experiment occurred（ P 〈 0.05） in the cytidine diphosphocholine group. Exogenous NGF treatment group showed significantly increased staining of ChAT-IR and anti-NGF, and changes in the labyrinthian experiment （ P 〈 0.01 ）. Conclusion Exogeneous NGF may improve the intelligenee of aged miee and inerease ChAT-IR and anti-NGF expression in various areas in the brain, and exert protective, supportive and reparative effects on neurons. |
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| Keywords: | Nerve Growth Factor （NGF） Dementia Choline aeetyltransterase （ChAT-IR） Senile miee |
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