| 鹭科和鸬鹚科卵黄抗体的纯化 |
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| 引用本文： | 杨帆,;林树柱,;刘先菊,;全雄志,;秦川,;张连峰.鹭科和鸬鹚科卵黄抗体的纯化[J].中国实验动物学杂志,2007(11):659-661. |
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| 作者姓名： | 杨帆 ;林树柱 ;刘先菊 ;全雄志 ;秦川 ;张连峰 |
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| 作者单位： | [1]中国医学科学院实验动物研究所北京协和医学院比较医学中心,北京100021 |
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| 基金项目： | 会公益研究专项（2005DIA1J095）. |
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| 摘 要： | 目的 纯化鹭科具有代表性的夜鹭及鸬鹚科具有代表性的鸬鹚的卵黄抗体IgY。方法 采用了水稀释法和硫酸铵分级沉淀法粗提IgY,再过HiTrap IgY Purification HP柱子进一步纯化。结果 经两步纯化,得到了纯化的鸬鹚和夜鹭的卵黄抗体IgY,经SDS-PAGE检测为电泳纯,夜鹭和鸬鹚的卵黄抗体的相对分子质量约为180×10^3。结论 证实了鸬鹚和夜鹭的卵黄抗体IgY的存在及其特性,为这两种鸟类的卵黄抗体IgY纯化,二级抗体制备提供了参考。
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| 关 键 词： | 鹭科 鸬鹚科 亲和层析 IgY |
Purification of IgY from Egg Yolk of Heron-family and Cormorant-family |
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| Institution: | YANG Fan, LIN Shu-zhu, QIN Chuan, LIU Xian-ju, QUAN Xiong-zhi, ZHANG Lian- feng ( Institute of Laboratory Animal Science, Chinese Academy of Medical Science ＆ Comparative Medicine Center, Peking Union Medical College, Beijing 100021 ) |
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| Abstract: | Objective To purify the IgY from egg yolk of night-heron and cormorant. Methods Egg yolk antibody was purified with water diluted method, ammonium sulfate precipitation and/or affinity chromatography. Results The purified products were identified by SDS-PAGE. The molecular weight of egg yolk antibody of night-heron and cormorant was 180 × 10^3 . Conclusion The purification method for IgY from night-heron and cormorant was established and this technique will be used to produce anti-IgY antibody and set up the immune-monitoring system for animals. |
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| Keywords: | Heron-family Cormorant-family IgY Atfinity chromatography |
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