Continuous hybridoma culture in a low-protein serum-free medium supplemented with liposomes |
| |
Authors: | Ad le Martial, Isabelle Gaillard, Jean-Marc Engasser,Annie Marc |
| |
Affiliation: | Laboratoire des Sciences du Génie Chimique, CNRS-INPL, Nancy Cedex, France |
| |
Abstract: | A homemade serum-free medium containing a low protein level under 0.1 g l−1 has been proved to support long-term cultures of VO 208 hybridoma cells successfully up to 50 days. The low protein level was achieved by supplying the lipids through liposomes containing cholesterol, oleic acid, - dipalmitoyl phosphatidylcholine, and bovine serum albumin. The influence of the liposome content in the feeding medium was studied in a continuous culture performed with step variations of the liposomes level, from 7.5 to 30 ml l−1. The cell density decreased at the highest liposomes content while it became higher with 7.5 or 12 ml l−1 of liposomes. For each step variation appeared a transitory activation of the specific rates of nutrient consumption, metabolite production and antibody secretion, as well as a transitory decrease of the specific cell growth rate. The overall structure of the antibodies was not affected during the culture. |
| |
Keywords: | Liposomes low-protein serum-free medium hybridoma cells continuous culture kinetics |
本文献已被 ScienceDirect 等数据库收录! |
|