Alteration of the enzymatic properties of smooth muscle myosin by a monoclonal antibody against subfragment 2. |
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Authors: | M Higashihara M Ikebe |
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Institution: | Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, OH 44106. |
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Abstract: | The fluorescent dye 10-N-nonyl acridine orange (NAO), known as specifically associated with mitochondria, has been reported to have a cytotoxic effect when high doses were applied to cells. Presently, the biochemical basis of its toxicity was investigated on isolated rat liver mitochondria. At low concentrations, NAO strongly inhibited state 3 respiration and ATP synthesis. At high concentrations, electron transport, ATP hydrolysis, Pi-transport and adenine nucleotide activities were also decreased. All these inhibitions can be explained by probe-cardiolipin interactions which could induce the collapse of energy conversion and/or the modification of membrane fluidity. |
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Keywords: | Nonyl acridine orange Respiratory chain mitochondria ATP synthesis (Rat liver mitochondria) |
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