Localization of VIP36 in the post-Golgi secretory pathway also of rat parotid acinar cells. |
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Authors: | Osamu Shimada Sayuri Hara-Kuge Katsuko Yamashita Hisami Tosaka-Shimada Li Yanchao Li Einan Saoko Atsumi Harunori Ishikawa |
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Institution: | Department of Anatomy, Yamanashi University School of Medicine, Yamanashi, Japan. oshimada@swallow.res.yamanashi-med.ac.jp |
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Abstract: | VIP36 (36-kD vesicular integral membrane protein), originally purified from Madin-Darby canine kidney (MDCK) epithelial cells, belongs to a family of animal lectins and may act as a cargo receptor. To understand its role in secretory processes, we performed morphological analysis of the rat parotid gland. Immunoelectron microscopy provided evidence that endogenous VIP36 is localized in the trans-Golgi network, on immature granules, and on mature secretory granules in acinar cells. Double-staining immunofluorescence experiments confirmed that VIP36 and amylase co-localized in the apical regions of the acinar cells. This is the first study to demonstrate that endogenous VIP36 is involved in the post-Golgi secretory pathway, suggesting that VIP36 plays a role in trafficking and sorting of secretory and/or membrane proteins during granule formation. |
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