Renaturation of dextranase activity from culture supernatant fluids of Streptococcus sobrinus after sodium dodecylsulfate polyacrylamide gel electrophoresis |
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| Authors: | J F Barrett R Curtiss |
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| Affiliation: | 1. State Key Laboratory of Explosion Science & Technology, Beijing Institute of Technology, No. 5, Zhongguancun South Street, Beijing 100081, China;2. Innovative Precision Machinery Manufacturing Co., Ltd. Fushun, Fushun 113103, China;1. Department of Pathology, Sun Yat-sen University Cancer Center, 651 Dongfeng Road East, Guangzhou 510060, China;2. Department of Neurosurgery/Neuro-oncology, Sun Yat-sen University Cancer Center, 651 Dongfeng Road East, Guangzhou 510060, China;3. Collaborative Innovation Center for Cancer Medicine, 651 Dongfeng Road East, Guangzhou 510060, China;4. State Key Laboratory of Oncology in South China, 651 Dongfeng Road East, Guangzhou 510060, China;1. Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran;2. Neuroscience Research Center, Poursina Hospital, Guilan University of Medical Sciences, Rasht, Iran;1. Instituto Politécnico Nacional, CIIDIR-Durango, 34220 Durango, Mexico;2. Facultad de Ciencias Químico-Biológicas, Universidad Autónoma de Sinaloa, 80040 Culiacán Rosales, Sinaloa, Mexico;3. Unidad Académica de Ciencias Químicas, Universidad Autónoma de Zacatecas, 98000 Zacatecas, Mexico;4. Hospital General de Durango, Secretaría de Salud Durango, 34000 Durango, Mexico;1. Normandie Univ, INSERM Unit 1073, Nutrition, Inflammation and Gut-Brain Axis, Rouen, France;2. University of Rouen, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France;3. Department of Gastroenterology, Rouen University Hospital, Rouen, France;4. Department of Nutrition, Rouen University Hospital, Rouen, France;1. State Key Laboratory of Genetic Engineering, Shanghai Engineering Research Center of Industrial Microorganisms, School of Life Science, Fudan University, Shanghai 200433, PR China;2. Shanghai Institute of Planned Parenthood Research Hospital, WHO Collaborating Center for Research in Human Reproduction, Shanghai 200433, PR China;3. State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, Shanghai 200433, PR China |
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| Abstract: | A rapid and reproducible method for the assay of individual fractions of the multicomponent dextranase activity of Streptococcus sobrinus after reduction/denaturation and electrophoresis in acrylamide gels is described. Multiple forms of dextranase, possible virulence factors in the formation of dental caries by oral Streptococci (S. mutans, S. sobrinus, S. sanguis, S. cricetus, and S. rattus), have been separated in sodium dodecylsulfate-polyacrylamide gels into which an indicator substrate, blue dextran, has been incorporated, and identified after renaturation to remove the reducing/denaturing agents of the Laemmli buffer system. |
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