Isolation and identification of lactic acid bacteria with effect of immune protection to Eschericia coli in mice |
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Authors: | Ishida-Fujii Keiko Goto Shingo Kuboki Hiroshi Hirano Shin-ichi Sakamoto Michiko Sato Michikatsu |
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Affiliation: | R & D Center, Alcohol Enterprise Head Office, New Energy and Industrial Technology Development Organization, 5-1, Inagehigashi 4-chome, Inage-ku, Chiba-shi, Chiba, 263-0031, Japan. fujii@jp-alcohol.com |
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Abstract: | Lactic acid bacteria were isolated from an alcohol fermentation broth, and the activity as a probiotic was examined using pathogenic E. coli. Thirty-six strains exhibiting good growth were isolated in the medium of concentrated mush which was a residue resulted in the alcohol distillation process. One of these strains, Lactobacillus paracasei subsp. paracasei I-5, could be grown in the medium containing 8 vol% ethanol and at 45 degrees C. The characteristics were different from the type strain, L. paracasei subsp. paracasei NBRC 15889. L. paracasei I-5 showed an excellent growth in the concentrated mush, which just diluted two-fold and adjusted the pH. ICR mice were fed with a standard germ-free feed (CMF) and the strain I-5 (7 x 10(9) cells/day) was orally administrated for 11 days prior to the intraperitoneal challenge with pathogenic E. coli Juhl. After the challenge, mice administrated the strain I-5 exhibited a high survival rate and survival extension days (p < 0.01) compared with the control. The results suggested that the strain might enhance the animal resistance against microbial pathogens. Neonatal diarrhea caused by E. coli is a serious disease in calf breeding. The strain might be practically valuable to prevent diarrhea in calves. |
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