| X射线对体外培养星形胶质细胞增生活化和分泌的影响 |
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| 引用本文： | 朱舟,汪琦,徐逸,王伟.X射线对体外培养星形胶质细胞增生活化和分泌的影响[J].中国组织化学与细胞化学杂志,2010,19(1):1-5. |
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| 作者姓名： | 朱舟 汪琦 徐逸 王伟 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院神经内科,武汉,430030 |
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| 基金项目： | 国家自然科学基金青年基金(30800343) |
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| 摘 要： | 目的观察X射线照射对培养星形胶质细胞(astrocyte,AS)增殖和分泌的影响。方法建立体外培养大鼠纯化的AS物理损伤模型(划痕损伤模型),分为对照组、划痕组和放疗组。利用免疫荧光观察胶质细胞胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)和5-溴脱氧尿嘧啶核苷(5-bromodeoxy uridine,BrdU)的表达,利用RT-PCR观察细胞因子IL-6和TNF-a的表达。结果划痕损伤刺激能使培养AS反应性增生活化。损伤后6h出现IL-6、TNF-a表达水平增高,24h后损伤周围BrdU阳性细胞率明显增加。通过X射线照射能抑制AS的BrdU表达,但并不能抑制损伤后IL-6和TNF-a的表达。结论X射线照射可以通过调控细胞周期,有效抑制损伤后AS的反应性增生,但不能对活化AS的IL-6和TNF-a的表达起到抑制作用。
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| 关 键 词： | 星形胶质细胞 X射线 增殖 活化 |
EFFECT OF X-RAY ON ASTROCYTE ACTIVATION AND SECRETION |
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| Zhu Zhou,Wang Qi,Xu Yi,Wang Wei.EFFECT OF X-RAY ON ASTROCYTE ACTIVATION AND SECRETION[J].Chinese Journal of Histochemistry and Cytochemistry,2010,19(1):1-5. |
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| Authors: | Zhu Zhou Wang Qi Xu Yi Wang Wei |
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| Institution: | Department of Neurology;Tongji Hospital;Tongji Medical College;Huazhong University of Science and Technology;Wuhan 430030;China |
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| Abstract: | Objective To observe the effect of X-ray on the proliferation and secretion of cultured astrocytes (AS). Methods The model of mechanical injury to cultured purified AS was established. The cultured cells were randomly divided into 3 equal groups: control group,scratch group (scratch + vehicle) and irradiation group (scratch + irradiation). Immunofluorescent (IF) staining was used to detect the expression of glial fibriliary acidic protein (GFAP) and 5-bromodeoxyuridine (BrdU). RT-PCR was used to detect the ... |
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| Keywords: | Astrocyte Cell cycle X-ray Proliferation Activation |
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