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Pivotal Role of the RB Family Proteins in in Vitro Thyroid Cell Transformation |
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| Authors: | Rodolfo Iuliano Francesco Trapasso Antonella Stella Ilaria Le Pera Rosa Marina Melillo Paola Bruni Gustavo Baldassarre Lorenzo Chiariotti Massimo Santoro Giuseppe Viglietto Alfredo Fusco |
| Affiliation: | a Dipartimento di Medicina Sperimentale e Clinica, Facoltà di Medicina e Chirurgia di Catanzaro, Università degli Studi di Catanzaro “Magna Græcia,” 88100, Catanzaro, Italy;b Centro di Endocrinologia ed Oncologia Sperimentale del C.N.R., c/o, Dipartimento di Biologia e Patologia Cellulare e Molecolare, Facoltà di Medicina e Chirurgia, Università di Napoli “Federico II,” 80131, Napoli, Italy;c Istituto dei Tumori di Napoli, Fondazione Pascale, via Mariano Semmola, 80131, Napoli, Italy |
| Abstract: | Rat thyroid differentiated cells (PC Cl 3) are an excellent model system with which to study the interaction between differentiation and cell transformation. We previously demonstrated that PC Cl 3 cells expressing the adenovirus E1A gene no longer depend on thyrotropin for growth and do not express thyroid differentiation markers. Here we show that an E1A mutant unable to bind the RB protein failed to transform the PC Cl 3 cells. Conversely, mutations in the E1A p300 interacting region did not affect its transforming ability. The pivotal role of RB family proteins in the thyroid cell transformation is supported by the thyrotropin independence induced by the E7 gene of human papilloma virus type 16, but not by a mutated form in the RB-binding region. |
| Keywords: | RB E1A E7 E2F thyroid transformation |
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