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Effect of substrate nitrogen on lignin degradation by Pleurotus ostreatus
Authors:Frank Commanday  Joan M Macy
Institution:(1) Department of Animal Science, University of California, 95616 Davis, CA, USA;(2) Present address: Oregon Graduate Center, 19600 NW Walker Rd., 97006 Beaverton, OR, USA
Abstract:In order to determine the effect of substrate nitrogen (N) on ligninolytic activity, Pleurotus ostreatus was grown in solid media containing either growth-limiting (1mM) or excess (10mM) NH4Cl. After 25 days, 14C–CO2 production from 14C-cornstover lignin in low-N medium was 3 times that in nitrogen (N)-rich medium. Supplementation of low-N medium with glucose (0.3%) further enhanced ligninolytic activity. Decolorization of an aromatic, polymeric dye, Poly R-481, in solid media was also greatest under N-limiting conditions.
Keywords:Pleurotus ostreatus  Poly R-481  Cinnamic acid  Biodegradation  Secondary Metabolism  Nitrogen Catabolite Repression  White Rot  Cornstover  Bioconversion  Lignin
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