Micropropagation of Aerides maculosum lindl. (Orchidaceae) |
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Authors: | H N Murthy A N Pyati |
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Institution: | (1) Department of Botany, Karnatak University, 580 003 Dharwad, India;(2) Present address: Center for the Development of Advanecd Horticultural Technology, Department of Horticulture, Chungbuk University, 361-763 Choengju, Korea |
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Abstract: | Summary Young leaf segments from plants growing both in vivo and in vitro were cultured on Murashige and Skoog (MS) medium supplemented with auxins naphthaleneacetic acid (NAA), 2,4-dichlorophenoxyacetic
acid (2,4-D)], cytokinins kinetin (KN) and N6-benzyladenine (BA)] and coconut liquid endosperm (CW). The explants from mature leaves did not show any growth and turned
necrotic, while those obtained from juvenile leaves growing in vitro developed protocorm-like bodies (PLBs) at their cut surfaces within 4–8 wk depending on the growth medium. An optimum of
18 PLBs developed from leaf explants on medium supplemented with 2.0 mg l−1 (8.87 μM) BA. Upon subculture in basal MS medium, the PLBs differentiated into plantlets within 6–8 wk. The resulting plantlets were
successfully transferred to vermiculite initially and subsequently to potting mixture; 84% of the plantlets survived after
3 mo. of transplantation. |
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Keywords: | orchid protocorm-like bodies (PLBs) Aerides maculosum |
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