DNA sequences required to induce localized conversion in Streptococcus pneumoniae transformation |
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Authors: | Pedro Garcia Anne-Marie Gasc Xenophon Kyriakidis Daniel Baty Michel Sicard |
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Affiliation: | (1) Centre de Recherche de Biochimie et Génétique Cellulaires du C.N.R.S., 118 route de Narbonne, 31062 F-Toulouse Cedex, France;(2) Centre de Biochimie et Biologie Moléculaire du C.N.R.S., 31 chemin Joseph Aiguier, BP 71, 13402 Marseille, France;(3) Present address: Centro de Investigacicnes Biológicas (CSIC), Velasquez 144, E-28006 Madrid, Spain |
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Abstract: | Summary In pneumococcal transformation a particular point mutation belonging to the amiA locus is able markedly to enhance recombination frequency when crossed with any other markers of this gene. This results from a polarized conversion of the mutation towards the wild-type sequence. In this report, by site-directed oligonucleotide mutagenesis, we have generated a series of mutants showing various degrees of conversion. We have found that the substitution 5-ATTCAT5-ATTAAT is a sufficient signal for localized conversion. Changing individual bases within this sequence results in decreased conversion frequencies to levels that depend on the mutation, suggesting that there is a family to related sequences which may act as a substrate for a conversion system. Moreover, the length over which this conversion occurs has been estimated to be 12 base pairs on the average. |
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Keywords: | Oligomer mutagenesis Mismatch repair Pneumococcus Recombination |
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