Electrophoresis of opioid peptides on columns with agarose suspension |
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Authors: | F Nyberg L Terenius |
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Institution: | Department of Pharmacology, University of Uppsala, S-751 23 Uppsala, Sweden |
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Abstract: | Opioid peptides have been fractionated by column electrophoresis in agarose suspension. The procedure provides a high resolution efficiency and compares favorably with high-performance liquid chromatography. Peptides were recovered from the column in 70% or higher yields when run in the femtomole to the nanomole range. By choosing different pHs it is possible to get complete resolution of a number of different endorphins. The electrophoreses were performed in volatile buffers, which are easily evaporated, and the residues are suitable for direct screening by radioreceptor- or radioimmunoassays. In combination with these assays the separation procedure makes it possible to detect and identify very low concentrations of endorphins in samples of biological origin. |
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Keywords: | agarose suspension electrophoresis endorphin enkephalin opioid peptides peptides |
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