Radioimmunoassay of main urinary metabolite of prostaglandin F2α |
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| Authors: | Shiro Ohki Katsuhiro Imaki Fumio Hirata Toshio Hanyu Nobuhiro Nakazawa |
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| Affiliation: | 1. Research Institute, Ono Pharmaceutical Co., Ltd. Sakurai, Shimamoto-Cho, Mishima-Gun, Osaka, Japan;2. Daiichi Radioisotope Lab., Ltd. 5-6-9, Narihira, Sumida-Ku, Tokyo, Japan |
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| Abstract: | Radioimmunoassay of 5α,7α-dihydroxy-11-keto-tetranorprosta-1,16-dioic acid, main urinary metabolite of prostaglandin F2α (PGF2α), was performed using an antiserum produced in the rabbit.The antibody in 100 μ1 of 1,600-fold diluted antiserum binds with 60 picograms of metabolite.The main urinary metabolite level fell when flufenamic acid, a prostaglandin synthetase inhibitor, was given to rats. In contrast, it was significantly elevated when PGF2α was administered. |
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